1100 µµmm64VVeerrssaattiillee DDiirreecctt LLaasseerr WWrriittiinngg Visualization of corrective motionSoftware analysis of molecular motors Micropatterns by DLWOrdered structure via self-organizationSimulation of self-organization behaviorDirect laser writing systemContent:ContentbymaterialVersatile Versatile direct laser writing, not limited by material photosensitivity,wasdevelopedusingultrashortpulselaserphotosensitivity, was developed using ultrashort pulse laser irradiation.Directlaserwritingisapowerfultoolfor3Dmicro-irradiation. Direct laser writing is a powerful tool for 3D /nanofabrication.However,therehasbeenintrinsiclimitationofmicro-/nanofabrication. However, there has been intrinsic materialchoicesofar.Namely,DLWcannotbeappliedtonon-limitation of material choice so far. Namely, DLW cannot be photosensitivematerials.Adequateopticalpropertiesoftargetapplied to non-photosensitive materials. Adequate optical materialareessentiallyrequired.Toovercomethislimitation,ourproperties of target material are essentially required. To grouphasstudiedaversatileDLW,whichisbasedonnonlinearovercome this limitation, our group has studied a versatile light-basedassemblyprocess.DLW, which is based on nonlinear light-based assembly ・Direct laser writing of functional materialsprocess. ・3D micro-/nanofabrication ・ Direct laser writing of functional materials・Laser-based assembly process in solution ・ 3D micro-/nanofabrication・Multi-modal soft micro-actuators for micromanipulation ・ Laser-based assembly process in solution ・ Multi-modal soft micro-actuators for micromanipulationAppealingpoint：Special objectivesWewelcomecollaborativeresearch,particularly,withexpertsofnanomaterialsandmicro-/nanoscience.Ourgrouphasawealthof We welcome collaborative research, particularly, with researchexperienceandresourcesonphoto-excitedphenomena.experts of nanomaterials and micro-/nanoscience. Our group has a wealth of research experience and resources on Yamagata UniversityGraduate School of Science and Engineering photo-excited phenomena. Research Interest ：Laser microfabrication, Nanophotonics, MEMSdirectContent：ContentActinandmyosinproteins,whicharefundamentalcomponentsfor Actin and myosin proteins, which are fundamental musclecontractions,carryoutefficientmovementwithamechano-components for muscle contractions, carry out efficient chemicalenergytransduction.Thesemotorproteinscanflexiblymovement with a mechano-chemical energy transduction. workonthenanometerscaledependingoncircumstance.WeThese motor proteins can flexibly work on the nanometer examinethemotilefunctionandmechano-chemicalcouplingofthescale depending on circumstance. We examine the motile motorproteinsbythemethodofreconstitutedmotilitysysteminfunction and mechano-chemical coupling of the motor vitro.Singeproteinfilamentsconjugatedwithdyescanbevisualizedbyfluorescencemicroscopy.Forthatanalysis,thedetectionaccuracyproteins by the method of reconstituted motility system in ofpositionofproteinsbasedonfluorescenceimageswasimprovedvitro. Singe protein filaments conjugated with dyes can be uptothenanometerscale.Furthermore,usingthefunctionsofmotorvisualized by fluorescence microscopy. For that analysis, the proteins,weattempttoconstructartificialorderedarchitecturesviadetection accuracy of position of proteins based on self-organizationandtocontrolanano-scaletransportsystem.fluorescence images was improved up to the nanometer scale. Furthermore, using the functions of motor proteins, we Appealing point：attempt to construct artificial ordered architectures via self-Proteinsarereferredtoas“molecularmachinery.”organization and to control a nano-scale transport system. elucidationofmechanismofnovelnano-technology.Special objectivesnotlaserwriting,limitedIbelievethattheirstructureandfunctionleadstoYamagata University Graduate School of Science and Engineering E-mail：nishiyama@yz.yamagata-u.ac.jpResearch InterestLaser microfabrication, Nanophotonics, MEMSTel ：+81-238-26-3210Fax：+81-238-26-3210E-mail ・ nishiyama@yz.yamagata-u.ac.jp Tel ・ +81-238-26-3210 Fax ・ +81-238-26-3210HP ：http://nishiyama-lab.yz.yamagata-u.ac.jp/index.htmlHP・http://nishiyama-lab.yz.yamagata-u.ac.jp/index.html Proteins are referred to as “molecular machinery.” I believe that elucidation of mechanism of their structure and Yamagata UniversityGraduate School of Science and Engineering function leads to novel nano-technology.Research Interest : Biophysics, Protein ScienceYamagata University Graduate School of Science and Engineering Research InterestE-mail: khatori@yz.yamagata-u.ac.jpBiophysics, Protein ScienceTel: +81-238-26-3727E-mail ・ khatori@yz.yamagata-u.ac.jpHP: http://khatori.yz.yamagata-u.ac.jp/publicationTel ・ ＋81-238-26-3727HP・http://khatori.yz.yamagata-u.ac.jp/publicationLaser Micro-/Nanofabrication & Optical MEMSLaser Micro-/Nanofabrication & Optical MEMSFunctional Analysis of Biological Molecular Motors Functional Analysis of Biological Molecular Motors Associate Professor Hiroaki NishiyamaAssoc. Prof. H. NishiyamaAssociate Professor Kuniyuki HatoriAssociate Professor KuniyukiHatori
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